Epigenetic clock and methylation studies in vervet monkeys
Anna J. Jasinska,
Joseph Alan Zoller,
Caesar Z Li,
Matthew J. Jorgensen,
Julie A. Mattison,
Andrew W. Rao,
Nelson B. Freimer,
Roger P. Woods,
Posted 10 Sep 2020
bioRxiv DOI: 10.1101/2020.09.09.289801
Posted 10 Sep 2020
DNA methylation-based biomarkers of aging have been developed for many mammals but not yet for the vervet monkey ( Chlorocebus sabaeus ), which is a valuable non-human primate model for biomedical studies. We generated novel DNA methylation data from vervet cerebral cortex, blood, and liver using highly conserved mammalian CpGs represented on a custom array (HorvathMammalMethylChip40). We present six DNA methylation-based estimators of age: vervet multi-tissue epigenetic clock and tissue-specific clocks for brain cortex, blood, and liver. In addition, two dual species clocks (human-vervet clocks) for measuring chronological age and relative age, respectively. Relative age was defined as ratio of chronological age to maximum lifespan to address the species differences in maximum lifespan. The high accuracy of the human-vervet clocks demonstrates that epigenetic aging processes are evolutionary conserved in primates. When applying these vervet clocks to tissue samples from another primate species, rhesus macaque, we observed high age correlations but strong offsets. We characterized CpGs that correlate significantly with age in the vervet. CpG probes hypermethylated with age across tissues were located near the targets of Polycomb proteins SUZ12 and EED, and genes possessing the trimethylated H3K27 mark in their promoters. The epigenetic clocks are expected to be useful for age estimation of wild-born animals and anti-aging studies in vervets. ### Competing Interest Statement SH is a founder of the non-profit Epigenetic Clock Development Foundation which plans to license several patents from his employer UC Regents. These patents list SH as inventor. The other authors declare no conflicts of interest.
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