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Acute noise causes down-regulation of ECM protein expression in guinea pig cochlea

By Min Shi, Lei Shi, Daxiong Ding, Yiyong Hu, Guowei Qi, Li Zhan, Yuhua Zhu, Ping Lv, Ning Yu

Posted 28 Sep 2020
bioRxiv DOI: 10.1101/2020.09.27.315952

Objective To study the differences in cochlea protein expression before and after noise exposure using proteomics to reveal the pathological mechanism of noise-induced hearing loss (NIHL). Methods A guinea pig NIHL model was established to test the ABR thresholds before and after noise exposure. The proteomics technology was used to study the mechanism of differential protein expression in the cochlea by noise stimulation. Results The average hearing threshold of guinea pigs on the first day after noise exposure was 57.00±6.78dB SPL, high-frequency hearing loss was more severe than low frequency; the average hearing threshold on the seventh day after noise exposure was 45.83±6.07dB SPL, and the 4k Hz hearing threshold has the best recovery. The proteomics technology identified 3122 different inner ear proteins, of which six proteins related to the hearing were down-regulation: TenascinC, Collagen type XI alpha two chains, Collagen type II alpha one chain, Thrombospondin 2, Collagen type XI alpha one chain and Ribosomal protein L38, and are enriched in protein absorption, focal adhesion, and extracellular matrix receptor pathways. Conclusion Impulse noise can affect the expression of differential proteins through focal adhesion pathways. This data can provide an experimental basis for the research on the prevention and treatment of NIHL. ### Competing Interest Statement The authors have declared no competing interest.

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