Rxivist logo

CRISPR/Cas12a mediated genome engineering in photosynthetic bacteria

By Yang Zhang, Jifeng Yuan

Posted 06 Oct 2020
bioRxiv DOI: 10.1101/2020.10.05.327569

Purple non-sulfur photosynthetic bacteria (PNSB) such as R. capsulatus serve as a versatile platform for fundamental studies and various biotechnological applications. In this study, we sought to develop the class II RNA-guided CRISPR/Cas12a system from Francisella novicida for both genome editing and gene down-regulation in R. capsulatus. About 90% editing efficiency was achieved by using CRISPR/Cas12a driven by a strong promoter Ppuc when targeting ccoO or nifH gene. When both genes were simultaneously targeted, the multiplex gene editing efficiency reached >63%. In addition, CRISPRi using deactivated Cas12a was also evaluated using reporter genes gfp and lacZ, and the repression efficiency reached >80%. In summary, our work represents the first report to develop CRISPR/Cas12a mediated genome editing/transcriptional repression in R. capsulatus, which would greatly accelerate PNSB-related researches.

Download data

  • Downloaded 381 times
  • Download rankings, all-time:
    • Site-wide: 130,597
    • In microbiology: 9,107
  • Year to date:
    • Site-wide: 121,805
  • Since beginning of last month:
    • Site-wide: 176,830

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News