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MiSiC, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities

By Swapnesh Panigrahi, Dorothée Murat, Antoine Le Gall, Eugénie Martineau, Kelly Goldlust, Jean-Bernard Fiche, Sara Rombouts, Marcelo Nöllmann, Leon Espinosa, Tâm Mignot

Posted 07 Oct 2020
bioRxiv DOI: 10.1101/2020.10.07.328666

Studies of microbial communities by live imaging require new tools for the robust identification of bacterial cells in dense and often inter-species populations, sometimes over very large scales. Here, we developed MiSiC, a general deep-learning-based segmentation method that automatically segments a wide range of spatially structured bacterial communities with very little parameter adjustment, independent of the imaging modality. Using a bacterial predator-prey interaction model, we demonstrate that MiSiC enables the analysis of interspecies interactions, resolving processes at subcellular scales and discriminating between species in millimeter size datasets. The simple implementation of MiSiC and the relatively low need in computing power make its use broadly accessible to fields interested in bacterial interactions and cell biology. ### Competing Interest Statement The authors have declared no competing interest.

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