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Alternate primers for whole-genome SARS-CoV-2 sequencing

By Matthew Cotten, Daniel Lule Bugembe, Pontiano Kaleebu, My V.T. Phan

Posted 12 Oct 2020
bioRxiv DOI: 10.1101/2020.10.12.335513

As the world is struggling to control the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), there is an urgency to develop effective control measures. Essential information is encoded in the virus genome sequence with accurate and complete SARS-CoV-2 sequences essential for tracking the movement and evolution of the virus and for guiding efforts to develop vaccines and antiviral drugs. While there is unprecedented SARS-CoV-2 sequencing efforts globally, approximately 19 to 43% of the genomes generated monthly are gapped, reducing their information content. The current study documents the genome gap frequencies and their positions in the currently available data and provides an alternative primer set and a sequencing scheme to help improve the quality and coverage of the genomes. ### Competing Interest Statement The authors have declared no competing interest.

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