Rapid Development of Neutralizing and Diagnostic SARS-COV-2 Mouse Monoclonal Antibodies
Asheley Poole Chapman,
Joo R. Lee,
Rebekah E. Wharton,
Markus H Kainulainen,
Jennifer L. Harcourt,
Roosecelis B. Martines,
Brigid C. Bollweg,
David E Wentworth,
Jason M. Goldstein
Posted 14 Oct 2020
bioRxiv DOI: 10.1101/2020.10.13.338095
Posted 14 Oct 2020
The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of the global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest is the ~300 amino acid receptor binding domain (RBD) within the S1 subunit of the spike protein because of its key interaction with the human angiotensin converting enzyme 2 (hACE2) receptor present on many cell types, especially lung epithelial cells. We report here the development and functional characterization of 29 nanomolar-affinity mouse SARS-CoV-2 mAbs created by an accelerated immunization and hybridoma screening process. Differing functions, including binding of diverse protein epitopes, viral neutralization, impact on RBD-hACE2 binding, and immunohistochemical staining of infected lung tissue, were correlated with variable gene usage and sequence. ### Competing Interest Statement The authors have declared no competing interest.
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