Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 73,697 bioRxiv papers from 320,714 authors.

Identification of piRNA binding sites reveals the Argonaute regulatory landscape of the C. elegans germline

By En-Zhi Shen, Hao Chen, Ahmet R. Ozturk, Shikui Tu, Masaki Shirayama, Wen Tang, Yue-He Ding, Si-Yuan Dai, Zhiping Weng, Craig C Mello

Posted 07 Feb 2018
bioRxiv DOI: 10.1101/262113 (published DOI: 10.1016/j.cell.2018.02.002)

piRNAs (Piwi-interacting small RNAs) engage Piwi Argonautes to silence transposons and promote fertility in animal germlines. Genetic and computational studies have suggested that C. elegans piRNAs tolerate mismatched pairing and in principle could target every transcript. Here we employ in vivo cross-linking to identify transcriptomewide interactions between piRNAs and target RNAs. We show that piRNAs engage all germline mRNAs and that piRNA binding follows microRNA-like pairing rules. Targeting correlates better with binding energy than with piRNA abundance, suggesting that piRNA concentration does not limit targeting. In mRNAs silenced by piRNAs, secondary small RNAs accumulate at the center and ends of piRNA binding sites. In germline-expressed mRNAs, however, targeting by the CSR-1 Argonaute correlates with reduced piRNA binding density and suppression of piRNA-associated secondary small RNAs. Our findings reveal physiologically important and nuanced regulation of individual piRNA targets and provide evidence for a comprehensive posttranscriptional regulatory step in germline gene expression.

Download data

  • Downloaded 883 times
  • Download rankings, all-time:
    • Site-wide: 10,683 out of 73,697
    • In genetics: 765 out of 4,041
  • Year to date:
    • Site-wide: 55,353 out of 73,697
  • Since beginning of last month:
    • Site-wide: 55,353 out of 73,697

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News