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Regulation of specific cell clusters in TCR-T cells responding to differential expression of tumor PD-L1

By Renpeng Ding, Shang Liu, Huanyi Chen, Bin Kang, Radoje Drmanac, Ying Gu, Xuan Dong, Qianqian Gao

Posted 29 Jul 2020
bioRxiv DOI: 10.1101/2020.07.28.224816 (published DOI: 10.34257/GJMRFVOL20IS8PG11)

PD-L1 signaling is important in regulating T cell function and keeping balance of tumor microenvironment, but its role in modifying TCR-T cell cytotoxicity remains unknown. MART-1-specific TCR-T cells (TCR-TMART-1) were stimulated by MEL-526 tumor cells expressing different proportions of PD-L1 and used to perform cytotoxicity assays and single-cell RNA sequencing. Percentage changes of different specific cell clusters were analyzed. The percentage of cluster HLA-DR+CD38+CD8+ was upregulated after antigen stimulation and tumor PD-L1 modified TCR-T cell function through downregulating the percentages of clusters HLA-DR+CD28+CD8+ and HLA-DR+CD38+CD8+ which were higher in TCR-TMART-1 than in Tnull. ### Competing Interest Statement The authors have declared no competing interest.

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