Rxivist logo

Regulation of specific cell clusters in TCR-T cells responding to differential expression of tumor PD-L1

By Renpeng Ding, Shang Liu, Huanyi Chen, Bin Kang, Radoje Drmanac, Ying Gu, Xuan Dong, Qianqian Gao

Posted 29 Jul 2020
bioRxiv DOI: 10.1101/2020.07.28.224816 (published DOI: 10.34257/GJMRFVOL20IS8PG11)

PD-L1 signaling is important in regulating T cell function and keeping balance of tumor microenvironment, but its role in modifying TCR-T cell cytotoxicity remains unknown. MART-1-specific TCR-T cells (TCR-TMART-1) were stimulated by MEL-526 tumor cells expressing different proportions of PD-L1 and used to perform cytotoxicity assays and single-cell RNA sequencing. Percentage changes of different specific cell clusters were analyzed. The percentage of cluster HLA-DR+CD38+CD8+ was upregulated after antigen stimulation and tumor PD-L1 modified TCR-T cell function through downregulating the percentages of clusters HLA-DR+CD28+CD8+ and HLA-DR+CD38+CD8+ which were higher in TCR-TMART-1 than in Tnull. ### Competing Interest Statement The authors have declared no competing interest.

Download data

  • Downloaded 93 times
  • Download rankings, all-time:
    • Site-wide: 144,992
    • In immunology: 4,328
  • Year to date:
    • Site-wide: 135,351
  • Since beginning of last month:
    • Site-wide: None

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News