A molecular pore spans the double membrane of the coronavirus replication organelle
Ronald W.A.L. Limpens,
Jessika C. Zevenhoven-Dobbe,
Anja W. M. de Jong,
Roman I. Koning,
David A. Agard,
Abraham J. Koster,
Eric J. Snijder,
Posted 25 Jun 2020
bioRxiv DOI: 10.1101/2020.06.25.171686 (published DOI: 10.1126/science.abd3629)
Posted 25 Jun 2020
Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored micro-environment for viral RNA synthesis in the infected cell. However, it is unclear how newly synthesized genomes and mRNAs can travel from these sealed replication compartments to the cytosol to ensure their translation and the assembly of progeny virions. Here, using cellular electron cryo-microscopy, we unveiled a molecular pore complex that spans both membranes of the double-membrane vesicle and would allow export of RNA to the cytosol. A hexameric assembly of a large viral transmembrane protein was found to form the core of the crown-shaped complex. This coronavirus-specific structure likely plays a critical role in coronavirus replication and thus constitutes a novel drug target. ### Competing Interest Statement The authors have declared no competing interest.
- Downloaded 1,405 times
- Download rankings, all-time:
- Site-wide: 10,265 out of 118,665
- In microbiology: 554 out of 9,371
- Year to date:
- Site-wide: 4,043 out of 118,665
- Since beginning of last month:
- Site-wide: 17,760 out of 118,665
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!