Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 62,232 bioRxiv papers from 276,305 authors.

Genetics of single-cell protein abundance variation in large yeast populations

By Frank W. Albert, Sebastian Treusch, Arthur H Shockley, Joshua S Bloom, Leonid Kruglyak

Posted 07 Nov 2013
bioRxiv DOI: 10.1101/000067 (published DOI: 10.1038/nature12904)

Many DNA sequence variants influence phenotypes by altering gene expression. Our understanding of these variants is limited by sample sizes of current studies and by measurements of mRNA rather than protein abundance. We developed a powerful method for identifying genetic loci that influence protein expression in very large populations of the yeast Saccharomyes cerevisiae. The method measures single-cell protein abundance through the use of green-fluorescent-protein tags. We applied this method to 160 genes and detected many more loci per gene than previous studies. We also observed closer correspondence between loci that influence protein abundance and loci that influence mRNA abundance of a given gene. Most loci cluster at hotspot locations that influence multiple proteins—in some cases, more than half of those examined. The variants that underlie these hotspots have profound effects on the gene regulatory network and provide insights into genetic variation in cell physiology between yeast strains.

Download data

  • Downloaded 629 times
  • Download rankings, all-time:
    • Site-wide: 13,899 out of 62,232
    • In genomics: 1,859 out of 4,277
  • Year to date:
    • Site-wide: 48,773 out of 62,232
  • Since beginning of last month:
    • Site-wide: 45,262 out of 62,232

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News