A thermostable, closed, SARS-CoV-2 spike protein trimer.
Katarzyna A Ciazynska,
Andrew P. Carter,
Guinevere L. Grice,
The CITIID-NIHR COVID-19 BioResource Collaboration,
James A Nathan,
Leo C James,
Helen E. Baxendale,
Ian G. Goodfellow,
John A. G. Briggs
Posted 17 Jun 2020
bioRxiv DOI: 10.1101/2020.06.15.152835 (published DOI: 10.1038/s41594-020-0478-5)
Posted 17 Jun 2020
The spike (S) protein of SARS-CoV-2 mediates receptor binding and cell entry and is the dominant target of the immune system. S exhibits substantial conformational flexibility. It transitions from closed to open conformations to expose its receptor binding site, and subsequently from prefusion to postfusion conformations to mediate fusion of viral and cellular membranes. S protein derivatives are components of vaccine candidates and diagnostic assays, as well as tools for research into the biology and immunology of SARS-CoV-2. Here we have designed mutations in S which allow production of thermostable, crosslinked, S protein trimers that are trapped in the closed, pre-fusion, state. We have determined the structures of crosslinked and non-crosslinked proteins, identifying two distinct closed conformations of the S trimer. We demonstrate that the designed, thermostable, closed S trimer can be used in serological assays. This protein has potential applications as a reagent for serology, virology and as an immunogen. ### Competing Interest Statement The authors have declared no competing interest.
- Downloaded 1,799 times
- Download rankings, all-time:
- Site-wide: 8,167
- In biochemistry: 159
- Year to date:
- Site-wide: 16,134
- Since beginning of last month:
- Site-wide: None
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!