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REALM: AO-based localization microscopy deep in complex tissue

By Marijn E. Siemons, Naomi A.K. Hanemaaijer, Maarten H.P. Kole, Lukas Kapitein

Posted 12 Jun 2020
bioRxiv DOI: 10.1101/2020.06.12.147884

Performing Single-Molecule Localization Microscopy (SMLM) in complex biological tissues, where sample-induced aberrations hamper detection and localization, has remained a challenge. Here we establish REALM (Robust and Effective Adaptive Optics in Localization Microscopy), which corrects aberrations of ≤1 rad RMS using 297 frames of blinking molecules to improve single-molecule localization. We demonstrate this method by resolving the periodic cytoskeleton of the axon initial segment at 50 μm depth in brain tissue. ### Competing Interest Statement The authors have declared no competing interest.

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