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Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction

By Ofir Israeli, Adi Beth-Din, Nir Paran, Dana Stein, Shirley Lazar, Shay Weiss, Elad Milrot, Yafit Atiya-Nasagi, Shmuel Yitzhaki, Orly Laskar, Ofir Schuster

Posted 10 Jun 2020
bioRxiv DOI: 10.1101/2020.06.10.144196 (published DOI: 10.1016/j.ijid.2020.08.015)

SARS-CoV-2 genetic identification is based on viral RNA extraction prior to RT-qPCR assay, however recent studies support the elimination of the extraction step. Herein, we assessed the RNA extraction necessity, by comparing RT-qPCR efficacy in several direct approaches vs. the gold standard RNA extraction, in detection of SARS-CoV-2 from laboratory samples as well as clinical Oro-nasopharyngeal SARS-CoV-2 swabs. Our findings show advantage for the extraction procedure, however a direct no-buffer approach might be an alternative, since it identified up to 70% of positive clinical specimens. ### Competing Interest Statement The authors have declared no competing interest.

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