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A method for continuously monitoring the quality of Masson pine seedlings

By Li Wan, Handong Gao, Zhuo Huang, Chao Li

Posted 01 Jun 2020
bioRxiv DOI: 10.1101/2020.06.01.127480

Root growth potential(RGP) is a popular physiological test method to evaluate seedling vigor. But the time used in RGP is the order of days , which lead to the poor performance of RGP. To decrease the time used on measuring RGP, we propose optical interference method, called statistical interferometry, to measure minute root elongation in the order of sub-nanometer, which is faster than RGP method with several orders of magnitude. Because we can measure the length of root elongation continuously, we can compute the root elongation rate(PER), which is the variety of root elongation's length per second. The continuously monitoring can help determine the masson seedling quality as soon as possible. To show the effectiveness of our proposed method, we designed an experiment, in which we applied different water stresses on our collected masson pine seedlings and acquired two groups of pines, representing two different quality: one is stressed by water and the other is not. After measuring PER of them in our experiments, we found that PER is interrelated with theĀ  quality of the seedlings.

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