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O-Pair Search with MetaMorpheus for O-glycopeptide Characterization

By Lei Lu, Nicholas McIlvain Riley, Michael R. Shortreed, Carolyn R Bertozzi, Lloyd M. Smith

Posted 20 May 2020
bioRxiv DOI: 10.1101/2020.05.18.102327 (published DOI: 10.1038/s41592-020-00985-5)

We report O-Pair Search, a new approach to identify O-glycopeptides and localize O-glycosites. Using paired collision- and electron-based dissociation spectra, O-Pair Search identifies O-glycopeptides using an ion-indexed open modification search and localizes O-glycosites using graph theory and probability-based localization. O-Pair Search reduces search times more than 2,000-fold compared to current O-glycopeptide processing software, while defining O-glycosite localization confidence levels and generating more O-glycopeptide identifications. O-Pair Search is freely available: https://github.com/smith-chem-wisc/MetaMorpheus. ### Competing Interest Statement The authors have declared no competing interest.

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