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Normalization of single-cell RNA-seq counts by log(x+1)* or log(1+x)*

By A. Sina Booeshaghi, Lior Pachter

Posted 19 May 2020
bioRxiv DOI: 10.1101/2020.05.19.100214

Single-cell RNA-seq technologies have been successfully employed over the past decade to generate many high resolution cell atlases. These have proved invaluable in recent efforts aimed at understanding the cell type specificity of host genes involved in SARS-CoV-2 infections. While single-cell atlases are based on well-sampled highly-expressed genes, many of the genes of interest for understanding SARS-CoV-2 can be expressed at very low levels. Common assumptions underlying standard single-cell analyses don’t hold when examining low-expressed genes, with the result that standard workflows can produce misleading results. Key Points ### Competing Interest Statement The authors have declared no competing interest.

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