CRISPR technologies have been widely adopted as powerful tools for targeted genomic manipulation. Recently, prime editing, a new CRISPR-based strategy for precision genome editing was developed that enables diverse genomic alterations to be directly written into target sites without requiring double-strand breaks (DSBs) or donor templates. While prime editing is a powerful tool for precision genome editing, the design of its unique prime editing guide RNAs (pegRNAs) is sophisticated for many users. Currently there is no automated tool exists for designing pegRNAs. We therefore developed pegFinder, a standalone algorithm that rapidly designs candidate pegRNAs. This tool enables quick and simple designing and ranking of pegRNAs for virtually any targeted loci simply by entering reference and desired edited sequences. We demonstrated the design with this tool, and showed in a case that among the outputs the top pegRNA recommended by pegFinder is functional in experimental validation. The web portal is available at (http://pegfinder.sidichenlab.org/). pegFinder thus represent the first available online tool for seamless design and recommendation of pegRNAs for diverse prime editing applications. ### Competing Interest Statement The authors have declared no competing interest.
- Downloaded 875 times
- Download rankings, all-time:
- Site-wide: 15,156 out of 94,912
- In bioengineering: 295 out of 2,155
- Year to date:
- Site-wide: 2,357 out of 94,912
- Since beginning of last month:
- Site-wide: 940 out of 94,912
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!