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Electron Event Representation (EER) data enables efficient cryoEM file storage with full preservation of spatial and temporal resolution

By Hui Guo, Erik Franken, Yuchen Deng, Samir Benlekbir, Garbi Singla Lezcano, Bart Janssen, Lingbo Yu, Zev A. Ripstein, Yong Zi Tan, John L Rubinstein

Posted 28 Apr 2020
bioRxiv DOI: 10.1101/2020.04.28.066795 (published DOI: 10.1107/S205225252000929X)

Direct detector device (DDD) cameras have revolutionized electron cryomicroscopy (cryoEM) with their high detective quantum efficiency (DQE) and output of movie data. A high ratio of camera frame rate (frames/sec) to camera exposure rate (electrons/pixel/sec) allows electron counting, which further improves DQE and enables recording of super-resolution information. Movie output also allows for computational correction of specimen movement and compensation for radiation damage. However, these movies come at the cost of producing large volumes of data. It is common practice to sum groups of successive camera frames to reduce the final frame rate, and therefore file size, to one suitable for storage and image processing. This reduction in the camera's temporal resolution requires decisions to be made during data acquisition that may result in the loss of information that could have been advantageous during image analysis. Here we present experimental analysis of a new Electron Event Representation (EER) data format for electron counting DDD movies, which is enabled by new hardware developed by Thermo Fisher Scientific for their Falcon DDD cameras. This format enables recording of DDD movies at the raw camera frame rate without sacrificing either spatial or temporal resolution. Experimental data demonstrate that the method retains super-resolution information and allows correction of specimen movement at the physical frame rate of the camera while maintaining manageable file sizes. The EER format will enable the development of new methods that can utilize the full spatial and temporal resolution of DDD cameras. ### Competing Interest Statement EF, YD, GSL, BJ, and LY are employees of Thermo Fisher Scientific. JLR is an advisor to Structura Biotechnology Inc.

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