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Rapid, sensitive, full genome sequencing of Severe Acute Respiratory Syndrome Virus Coronavirus 2 (SARS-CoV-2)

By Clinton R Paden, Ying Tao, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, Suxiang Tong

Posted 24 Apr 2020
bioRxiv DOI: 10.1101/2020.04.22.055897 (published DOI: 10.3201/eid2610.201800)

SARS-CoV-2 recently emerged, resulting a global pandemic. Rapid genomic information is critical to understanding transmission and pathogenesis. Here, we describe validated protocols for generating high-quality full-length genomes from primary samples. The first employs multiplex RT-PCR followed by MinION or MiSeq sequencing. The second uses singleplex, nested RT-PCR and Sanger sequencing. ### Competing Interest Statement The authors have declared no competing interest.

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