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Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients

By Christian Ihling, Dirk Tänzler, Sven Hagemann, Astrid Kehlen, Stefan Huettelmaier, Andrea Sinz

Posted 19 Apr 2020
bioRxiv DOI: 10.1101/2020.04.18.047878 (published DOI: 10.1021/acs.jproteome.0c00280)

Mass spectrometry (MS) can deliver valuable diagnostic data that complements genomic information and allows us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. ### Competing Interest Statement The authors have declared no competing interest.

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