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Precise, high-throughput production of multicellular spheroids with a bespoke 3D bioprinter

By Robert H Utama, Lakmali Atapattu, Aidan P. O’Mahony, Christopher M Fife, Jongho Baek, Théophile Allard, Kieran J. O’Mahony, Julio Ribeiro, Katharina Gaus, Maria Kavallaris, J. Justin Gooding

Posted 07 Apr 2020
bioRxiv DOI: 10.1101/2020.04.06.028548

3D in vitro cancer models are important therapeutic and biological discovery tools, yet formation of multicellular spheroids in a throughput and highly controlled manner to achieve robust and statistically relevant data, remains challenging. Here, we developed an enabling technology consisting of a bespoke drop-on-demand 3D bioprinter capable of high-throughput printing of 96-well plates of spheroids. 3D-multicellular spheroids are embedded inside a tissue-like matrix with precise control over size and cell number. Application of 3D bioprinting for high-throughput drug screening was demonstrated with doxorubicin. Measurements showed that IC50 values were sensitive to spheroid size, embedding and how spheroids conform to the embedding, revealing parameters shaping biological responses in these models. Our study demonstrates the potential of 3D bioprinting as a robust high-throughput platform to screen biological and therapeutic parameters. ### Competing Interest Statement A.P.O.M, T.A., K.J.O.M and J.R. are employees and/or consultants of Inventia Life Science Pty Ltd. Inventia Life Science Pty Ltd has an interest in commercializing the technology.

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