Single Molecule Sequencing Of M13 Virus Genome Without Amplification
Posted 03 May 2017
bioRxiv DOI: 10.1101/133843 (published DOI: 10.1371/journal.pone.0188181)
Posted 03 May 2017
Third generation sequencing is a direct measurement of DNA/RNA sequences at the single molecule level without amplification. In this study, we report sequencing of the genome of the M13 virus by a new single molecule sequencing platform. Our platform detects single molecule fluorescence by the total internal reflection microscope technique, with sequencing-by-synthesis chemistry. We sequenced the genome of M13 to a depth of 316x and 100% coverage. The consensus sequence accuracy is 100%. We demonstrated that single molecule sequencing has no significant GC bias.
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