Rxivist logo

PacBio SMRT-based full-length transcriptome data of Ascosphaera apis mycelium and spore

By Yu Du, Jie Wang, Huazhi Chen, Xiaoxue Fan, Zhiwei Zhu, Yuanchan Fan, Haibin Jiang, Cuiling Xiong, Yanzhen Zheng, Dafu Chen, Rui Guo

Posted 20 Mar 2020
bioRxiv DOI: 10.1101/2020.03.19.998625

Ascosphaera apis is an entomopathogenic fungus that exclusively infects honeybee larvae, resulting in chalkbrood disease, a widespread fungal disease damaging the beekeeping industry all over the world. In this article, purified mycelia (Aam) and spores (Aas) of A. apis pure culture under lab condition were sequenced using PacBio Sequel platform. In total, 13,302,489 and 9,911,345 subreads were yielded from Aam and Aas, respectively; 394,142 and 274,928 circular consensus sequence (CCS) reads were identified as being full-length non-chimeric (FLNC) reads, with a mean length of 2820 bp and 2602 bp, respectively. Furthermore, 174,095 and 103,845 corrected isoforms were identified, with a N50 length of 3543 bp and 3262 bp, respectively. The reported full-length transcriptome data of A. apis mycelium and spore will provide a valuable resource for improvement of genome and transcriptome annotations as well as better understanding of transcript structure such as alternative splicing and polyadenylation.

Download data

  • Downloaded 287 times
  • Download rankings, all-time:
    • Site-wide: 158,031
    • In evolutionary biology: 7,093
  • Year to date:
    • Site-wide: 177,559
  • Since beginning of last month:
    • Site-wide: 161,583

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide