Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 60,240 bioRxiv papers from 267,843 authors.
Large-scale simultaneous measurement of epitopes and transcriptomes in single cells.
Pratip K Chattopadhyay,
Posted 02 Mar 2017
bioRxiv DOI: 10.1101/113068 (published DOI: 10.1038/nmeth.4380)
Posted 02 Mar 2017
Recent high-throughput single-cell sequencing approaches have been transformative for understanding complex cell populations, but are unable to provide additional phenotypic information, such as protein levels of cell-surface markers. Using oligonucleotide-labeled antibodies, we integrate measurements of cellular proteins and transcriptomes into an efficient, sequencing-based readout of single cells. This method is compatible with existing single-cell sequencing approaches and will readily scale as the throughput of these methods increase.
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