Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 62,734 bioRxiv papers from 278,354 authors.

Questioning unexpected CRISPR off-target mutations in vivo

By Sang-Tae Kim, Jeongbin Park, Daesik Kim, Kyoungmi Kim, Sangsu Bae, Matthias Schlesner, Jin-Soo Kim

Posted 30 Jun 2017
bioRxiv DOI: 10.1101/157925 (published DOI: 10.1038/nmeth.4554)

In summary, the CRISPR-Cas9-treated mice do not exhibit more variants that are absent in the uncorrected control than vice versa. Furthermore, the observed variants are extremely unlikely a consequence of a mutagenic process in the investigated animal, but are much better explained by differences in the genetic background between the two gene-edited mice and the control mouse. We conclude that the data presented by Schaefer et al does not provide any evidence for off-target mutagenesis due to CRISPR-Cas9 treatment and see no reason to be doubtful about the previous findings that Cas9 does not cause SNVs and indels at non-homologous, PAM-free sites.

Download data

  • Downloaded 1,340 times
  • Download rankings, all-time:
    • Site-wide: 4,381 out of 62,734
    • In genomics: 790 out of 4,316
  • Year to date:
    • Site-wide: 39,833 out of 62,734
  • Since beginning of last month:
    • Site-wide: 35,262 out of 62,734

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)