Rxivist logo

Questioning unexpected CRISPR off-target mutations in vivo

By Sang-Tae Kim, Jeongbin Park, Daesik Kim, Kyoungmi Kim, Sangsu Bae, Matthias Schlesner, Jin-Soo Kim

Posted 30 Jun 2017
bioRxiv DOI: 10.1101/157925 (published DOI: 10.1038/nmeth.4554)

In summary, the CRISPR-Cas9-treated mice do not exhibit more variants that are absent in the uncorrected control than vice versa. Furthermore, the observed variants are extremely unlikely a consequence of a mutagenic process in the investigated animal, but are much better explained by differences in the genetic background between the two gene-edited mice and the control mouse. We conclude that the data presented by Schaefer et al does not provide any evidence for off-target mutagenesis due to CRISPR-Cas9 treatment and see no reason to be doubtful about the previous findings that Cas9 does not cause SNVs and indels at non-homologous, PAM-free sites.

Download data

  • Downloaded 1,411 times
  • Download rankings, all-time:
    • Site-wide: 6,074 out of 84,643
    • In genomics: 993 out of 5,463
  • Year to date:
    • Site-wide: 66,067 out of 84,643
  • Since beginning of last month:
    • Site-wide: 59,974 out of 84,643

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News