Rxivist logo

Hippo signaling promotes Ets21c-dependent apical cell extrusion in the Drosophila wing disc

By Xianlong Ai, Dan Wang, Junzheng Zhang, Jie Shen

Posted 04 Mar 2020
bioRxiv DOI: 10.1101/2020.03.03.975771 (published DOI: 10.1242/dev.190124)

Cell extrusion is a crucial regulator of epithelial tissue development and homeostasis. Epithelial cells undergoing apoptosis, bearing pathological mutations, and possessing developmental defects are actively extruded toward elimination. However, the molecular mechanisms of Drosophila epithelial cell extrusion are not fully understood. Here, we report that activation of the conserved Hippo (Hpo) signaling pathway induces both apical and basal cell extrusion in the Drosophila wing disc epithelia. We show that canonical Yorki targets Diap1, and that dMyc and Cyclin E are not required for either apical or basal cell extrusion induced by activation of this pathway. Another target gene, bantam, is only involved in basal cell extrusion, suggesting novel Hpo-regulated apical cell extrusion mechanisms. Using RNA-Seq analysis, we found that JNK signaling is activated in the extruding cells. We provide genetic evidence that JNK signaling activation is both sufficient and necessary for Hpo-regulated cell extrusion. Furthermore, we demonstrate that the ETS-domain transcription factor Ets21c, an ortholog of proto-oncogenes FLI1 and ERG, acts downstream of JNK signaling to mediate apical cell extrusion. Our findings reveal a novel molecular link between Hpo signaling and cell extrusion.

Download data

  • Downloaded 353 times
  • Download rankings, all-time:
    • Site-wide: 94,111
    • In developmental biology: 2,529
  • Year to date:
    • Site-wide: 83,501
  • Since beginning of last month:
    • Site-wide: 77,129

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News