Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 72,923 bioRxiv papers from 317,485 authors.

A Mechanism of Cohesin-Dependent Loop Extrusion Organizes Zygotic Genome Architecture

By Johanna Gassler, Hugo B. Brandão, Maxim Imakaev, Ilya M. Flyamer, Sabrina Ladstätter, Wendy A. Bickmore, Jan-Michael Peters, Leonid A. Mirny, Kikuë Tachibana-Konwalski

Posted 17 Aug 2017
bioRxiv DOI: 10.1101/177766 (published DOI: 10.15252/embj.201798083)

Fertilization triggers assembly of higher-order chromatin structure from a naive genome to generate a totipotent embryo. Chromatin loops and domains are detected in mouse zygotes by single-nucleus Hi-C (snHi-C) but not bulk Hi-C. We resolve this discrepancy by investigating whether a mechanism of cohesin-dependent loop extrusion generates zygotic chromatin conformations. Using snHi-C of mouse knockout embryos, we demonstrate that the zygotic genome folds into loops and domains that depend on Scc1-cohesin and are regulated in size by Wapl. Remarkably, we discovered distinct effects on maternal and paternal chromatin loop sizes, likely reflecting loop extrusion dynamics and epigenetic reprogramming. Polymer simulations based on snHi-C are consistent with a model where cohesin locally compacts chromatin and thus restricts inter-chromosomal interactions by active loop extrusion, whose processivity is controlled by Wapl. Our simulations and experimental data provide evidence that cohesin-dependent loop extrusion organizes mammalian genomes over multiple scales from the one-cell embryo onwards.

Download data

  • Downloaded 1,306 times
  • Download rankings, all-time:
    • Site-wide: 5,509 out of 72,924
    • In genomics: 941 out of 4,843
  • Year to date:
    • Site-wide: 54,519 out of 72,924
  • Since beginning of last month:
    • Site-wide: 54,519 out of 72,924

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)