Rxivist logo

In-cell architecture of an actively transcribing-translating expressome

By Francis J. O’Reilly, Liang Xue, Andrea Graziadei, Ludwig R Sinn, Swantje Lenz, Dimitry Tegunov, Cedric Blötz, Wim J. H. Hagen, Patrick Cramer, Jörg Stülke, Julia Mahamid, Juri Rappsilber

Posted 28 Feb 2020
bioRxiv DOI: 10.1101/2020.02.28.970111 (published DOI: 10.1126/science.abb3758)

Structural biology performed inside cells can capture molecular machines in action within their native context. Here we develop an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae. We combine whole-cell crosslinking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at sub-nanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpoint NusA at the interface between a NusG-bound elongating RNAP and the ribosome, and propose it could mediate transcription-translation coupling. Translation inhibition dissociates the expressome, whereas transcription inhibition stalls and rearranges it, demonstrating that the active expressome architecture requires both translation and transcription elongation within the cell.

Download data

  • Downloaded 3,880 times
  • Download rankings, all-time:
    • Site-wide: 3,672
    • In molecular biology: 76
  • Year to date:
    • Site-wide: 37,766
  • Since beginning of last month:
    • Site-wide: 42,686

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News