An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
M. Ryan Corces,
Alexandro E. Trevino,
Emily G. Hamilton,
Peyton G Greenside,
Nicholas A. Sinnott-Armstrong,
Ansuman T. Satpathy,
Adam J. Rubin,
Kathleen S. Montine,
Seung Woo Cho,
Maxwell R. Mumbach,
Ava C. Carter,
Lisa A. Orloff,
Viviana I Risca,
Paul A. Khavari,
Thomas J. Montine,
William J. Greenleaf,
Howard Y. Chang
Posted 26 Aug 2017
bioRxiv DOI: 10.1101/181206 (published DOI: 10.1038/nmeth.4396)
Posted 26 Aug 2017
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol enables chromatin accessibility profiling from archival frozen tissue samples and 50 um sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
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