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Antagonistic interactions subdue inter-species green-beard cooperation in bacteria.

By Santosh Sathe, Rolf Kümmerli

Posted 26 Feb 2020
bioRxiv DOI: 10.1101/2020.02.25.965665 (published DOI: 10.1111/jeb.13666)

Cooperation can be favored through the green-beard mechanism, where a set of linked genes encodes both a cooperative trait and a phenotypic marker (green beard), which allows carriers of the trait to selectively direct cooperative acts to other carriers. In theory, the green-beard mechanism should favor cooperation even when interacting partners are totally unrelated at the genome level. Here, we explore such an extreme green-beard scenario between two unrelated bacterial species − Pseudomonas aeruginosa and Burkholderia cenocepacia , which share a cooperative locus encoding the public good pyochelin (a siderophore) and its cognate receptor (green beard) required for iron-pyochelin uptake. We show that pyochelin, when provided in cell-free supernatants, can be mutually exchanged between species and provide fitness benefits under iron limitation. However, in co-culture we observed that these cooperative benefits vanished and communities were dominated by P. aeruginosa , regardless of strain background and species starting frequencies. Our results further suggest that P. aeruginosa engages in interference competition to suppress B. cenocepacia , indicating that inter-species conflict arising from dissimilarities at the genome level overrule the aligned cooperative interests at the pyochelin locus. Thus, green-beard cooperation is subdued by competition, indicating that inter-specific siderophore cooperation is difficult to evolve and to be maintained.

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