Rxivist logo

Large-scale Quantification of Vertebrate Biodiversity in Ailaoshan Nature Reserve from Leech iDNA

By Yinqiu Ji, Christopher CM Baker, Yuanheng Li, Viorel D. Popescu, Zhengyang Wang, Jiaxin Wang, Lin Wang, Chunying Wu, Chaolang Hua, Zhongxing Yang, Chunyan Yang, Charles C.Y. Xu, Qingzhong Wen, Naomi E Pierce, Douglas W. Yu

Posted 11 Feb 2020
bioRxiv DOI: 10.1101/2020.02.10.941336

Environmental DNA (eDNA) has great potential to complement visual surveys, camera trapping, and bioacoustics in measuring biodiversity. We report here a large-scale attempt to use DNA from leech-ingested bloodmeals to estimate vertebrate occupancy at the scale of an entire protected area: the 677 km2 Ailaoshan national-level nature reserve in Yunnan province, southwest China. We contracted 163 park rangers to collect leeches in 172 patrol areas, resulting in 30,468 total leeches, divided over 893 replicate samples. Replicate sampling from each patrol area was achieved by providing rangers with preservative-filled tubes in separate, self-sealing bags, inducing them to distribute collected leeches over multiple bags, which we could sequence separately. We show that combining leech-derived DNA data with Bayesian site-occupancy modeling can produce detailed and useful biodiversity inferences for a wide range of vertebrates in a reasonable timeframe. For example, in Ailaoshan, sites at lower elevations and closer to the reserve edge have higher occupancy of domestic species (cows, sheep, goats) compared to sites at higher elevations and closer to the reserve interior. Muntjak deer show similar occupancy patterns to the domesticated species, but otherwise, most species of large mammal wildlife (e.g. sambar, black bear, serow, tufted deer) show the opposite pattern: greater occupancy at higher elevations and closer to the reserve interior. We conclude that leech-derived eDNA can be used to efficiently measure the effectiveness of protected areas in terms of vertebrate biodiversity outcomes and to help us optimize the deployment of management resources within reserves, by providing valuable information on the spatial distributions of vertebrate species and on the environmental and anthropogenic correlates of those distributions.

Download data

  • Downloaded 505 times
  • Download rankings, all-time:
    • Site-wide: 36,050 out of 100,936
    • In ecology: 1,063 out of 4,415
  • Year to date:
    • Site-wide: 7,439 out of 100,936
  • Since beginning of last month:
    • Site-wide: 8,068 out of 100,936

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News

  • 20 Oct 2020: Support for sorting preprints using Twitter activity has been removed, at least temporarily, until a new source of social media activity data becomes available.
  • 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
  • 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
  • 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
  • 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
  • 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
  • 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
  • 22 Jan 2019: Nature just published an article about Rxivist and our data.
  • 13 Jan 2019: The Rxivist preprint is live!