Rxivist logo

Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.

Download data

  • Downloaded 3,942 times
  • Download rankings, all-time:
    • Site-wide: 3,889
    • In neuroscience: 272
  • Year to date:
    • Site-wide: 4,286
  • Since beginning of last month:
    • Site-wide: 4,517

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide