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Gut lymph purification regulates monocyte activity in rats with ischemia-reperfusion injury-induced sepsis

By Wei Zhang, Jie Chen, Can Jin, Shuncheng Zhang, Juan Gu, Meimei Shi

Posted 02 Feb 2020
bioRxiv DOI: 10.1101/2020.02.02.930610

Objective: To confirm that gut lymph purification (GLP) based on oXiris regulates monocyte activity by targeting the removal of ischemia-reperfusion injury (IRI)-induced intestinal toxic substances (ITSs) in rats. Methods: Sepsis was induced by intestinal IRI in 24 adult male Sprague-Dawley rats that were randomly divided into the control, intestinal IRI, and IRI+GLP groups. The gut lymph fluid (GLF) was drained for 180 minutes. The ITSs levels and the proliferation, apoptosis and positive expression rates of MHC-II molecules of monocytes coincubated with the GLF were detected. Results: Endotoxin, TNF-α, IL-4, IL-6 and IL-10 levels in the lymph and plasma of the IRI group were significantly higher than those of the control group (p < 0.01). Compared with the IRI group, GLP treatment significantly decreased the ITS levels (p < 0.05). Monocyte proliferation and the positive expression rate of MHC-Ⅱ molecules were significantly reduced after co-culturing with GLF upon IRI (p < 0.01), and the apoptotic rate was significantly increased (p < 0.01). However, culturing monocytes with GLP significantly enhanced the monocyte proliferation, increased the positive expression rate of MHC-Ⅱ monocytes (p < 0.01), and reduced the apoptotic rate (p < 0.01). Conclusions: GLP therapy based on oXiris effectively removed ITSs from the GLF after IRI, thereby blocking the main process of multiple organ dysfunction syndrome by regulating monocyte activity.

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