Rxivist logo

The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We utilised single-cell RNA-sequencing (scRNA-seq) to create a cell census of the human thymus and to reconstruct T-cell differentiation trajectories and T-cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ novel CD8aa+ T-cell populations, thymic fibroblast subtypes and activated dendritic cell (aDC) states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and suggests later commitment of the CD8+ T-cell lineage. Taken together, our data provide a comprehensive atlas of the human thymus across the lifespan with new insights into human T-cell development.

Download data

  • Downloaded 4,772 times
  • Download rankings, all-time:
    • Site-wide: 2,509
    • In immunology: 84
  • Year to date:
    • Site-wide: 3,227
  • Since beginning of last month:
    • Site-wide: 105,267

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide