Rxivist logo

In Silico Modeling of Metabolic State in Single Th17 Cells Reveals Novel Regulators of Inflammation and Autoimmunity

By Allon Wagner, Chao Wang, David DeTomaso, Julian Avila-Pacheco, Sarah Zaghouani, Johannes Fessler, Sequoia Eyzaguirre, Elliot Akama-Garren, Kerry Pierce, Noga Ron-Harel, Vivian Paraskevi Douglas, Marcia Haigis, Raymond A. Sobel, Clary Clish, Aviv Regev, Vijay K Kuchroo, Nir Yosef

Posted 24 Jan 2020
bioRxiv DOI: 10.1101/2020.01.23.912717

Cellular metabolism, a key regulator of immune responses, is difficult to study with current technologies in individual cells Here, we present Compass, an algorithm to characterize the metabolic state of cells based on single-cell RNA-Seq and flux balance analysis. We applied Compass to associate metabolic states with functional variability (pathogenic potential) amongst Th17 cells and recovered a metabolic switch between glycolysis and fatty acid oxidation, akin to known differences between Th17 and Treg cells, as well as novel targets in amino-acid pathways, which we tested through targeted metabolic assays. Compass further predicted a particular glycolytic reaction (phosphoglycerate mutase - PGAM) that promotes an anti-inflammatory Th17 phenotype, contrary to the common understanding of glycolysis as pro-inflammatory. We demonstrate that PGAM inhibition leads non-pathogenic Th17 cells to adopt a pro-inflammatory transcriptome and induce autoimmunity in vivo. Compass is broadly applicable for characterizing metabolic states of cells and relating metabolic heterogeneity to other cellular phenotypes.

Download data

  • Downloaded 550 times
  • Download rankings, all-time:
    • Site-wide: 23,141 out of 78,244
    • In genomics: 2,565 out of 5,120
  • Year to date:
    • Site-wide: 988 out of 78,244
  • Since beginning of last month:
    • Site-wide: 1,362 out of 78,244

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News