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Tiled Reconstruction Improves Structured Illumination Microscopy

By David P. Hoffman, Eric Betzig

Posted 06 Jan 2020
bioRxiv DOI: 10.1101/2020.01.06.895318

Structured illumination microscopy (SIM) is widely used for fast, long-term, live-cell super-resolution imaging. However, SIM images can contain substantial artifacts if the sample does not conform to the underlying assumptions of the reconstruction algorithm. Here we describe a simple, easy to implement, process that can be combined with any reconstruction algorithm to alleviate many common SIM reconstruction artifacts and briefly discuss possible extensions.

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