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Enhancing generating and collecting efficiency of single particle upconverting luminescence at low-level power excitation

By Chenshuo Ma, Chunyan Shan, Kevin Park, Aaron T Mok, Xusan Yang

Posted 18 Dec 2019
bioRxiv DOI: 10.1101/2019.12.17.879858 (published DOI: 10.1515/nanoph-2019-0526)

Upconverting luminescent nanoparticles are photostable, non-blinking, and low chemically toxic fluorophores that are emerging as promising fluorescent probe at single-molecule level. High luminescence intensity upconversion nanoparticles (UCNPs) is achieved with highly doped rare-earth ions co-doped (20% Yb3+) using high excitation power (>2.5 MW/cm2). However, such particles are inadequate for in-vitro live-cell imaging and single-particle tracking since high excitation power can cause photodamage. Here, we compared UCNPs luminescence intensities with different dopants concentrations and presented a more efficient (~7x) UCNPs at low excitation power by increasing the concentrations of Yb3+ and Tm3+ dopants (NaYF4: 60% Yb3+, 8% Tm3+) and adding a core-shell structure.

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