Rxivist logo

Competition between kinesin-1 and myosin-V define Drosophila posterior determination

By Wen Lu, Margot Lakonishok, Rong Liu, Neil Billington, Ashley Rich, Michael Glotzer, James R. Sellers, Vladimir I Gelfand

Posted 05 Dec 2019
bioRxiv DOI: 10.1101/866467 (published DOI: 10.7554/eLife.54216)

Local accumulation of oskar (osk) mRNA in the Drosophila oocyte determines the posterior pole of the future embryo. Two major cytoskeletal components, microtubules and actin filaments, together with a microtubule motor, kinesin-1, and an actin motor, myosin-V, are essential for osk mRNA posterior localization. In this study, we use Staufen, an RNA-binding protein that colocalizes with osk mRNA, as a proxy for posterior determination. We demonstrate that posterior localization of osk/Staufen is determined by competition between kinesin-1 and myosin-V. While kinesin-1 removes osk/Staufen from the cortex along microtubules, myosin-V anchors osk/Staufen at the cortex. Myosin-V wins over kinesin-1 at the posterior pole due to low microtubule density at this site, while kinesin-1 wins it at anterior and lateral positions because they have high density of cortically-anchored microtubules. As a result, posterior determinants are removed from the anterior and lateral cortex but retained at the posterior pole. Thus, posterior determination of Drosophila oocyte is defined by kinesin-myosin competition, whose outcome is primarily determined by cortical microtubule density.

Download data

  • Downloaded 325 times
  • Download rankings, all-time:
    • Site-wide: 100,190
    • In cell biology: 4,621
  • Year to date:
    • Site-wide: 152,002
  • Since beginning of last month:
    • Site-wide: 149,481

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide