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TORC1 regulates vacuole membrane composition through ubiquitin- and ESCRT-dependent microautophagy

By Xi Yang, Weichao Zhang, Xin Wen, Patrick J. Bulinski, Dominic A. Chomchai, Felichi Mae Arines, Yun-Yu Liu, Simon Sprenger, David Teis, Daniel J. Klionsky, Ming Li

Posted 26 Nov 2019
bioRxiv DOI: 10.1101/854760 (published DOI: 10.1083/jcb.201902127)

Cellular adaptation in response to nutrient limitation requires the induction of autophagy and lysosome biogenesis for the efficient recycling of macromolecules. Here, we discovered that starvation and TORC1 inactivation not only lead to the upregulation of autophagy and vacuole proteins involved in recycling, but also result in the downregulation of many vacuole membrane proteins to supply amino acids as part of a vacuole remodeling process. Downregulation of vacuole membrane proteins is initiated by ubiquitination, which is accomplished by the coordination of multiple E3 ubiquitin ligases, including Rsp5, the Dsc complex, and a newly characterized E3 ligase, Pib1. The Dsc complex is negatively regulated by TORC1 through the Rim15-Ume6 signaling cascade. After ubiquitination, vacuole membrane proteins are sorted into the lumen for degradation by ESCRT-dependent microautophagy. Thus, our study uncovered a complex relationship between TORC1 inactivation and vacuole biogenesis.

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