Mapping polyclonal antibody responses in non-human primates vaccinated with HIV Env trimer subunit vaccines
Christopher A Cottrell,
Robert N. Kirchdoerfer,
Leigh M. Sewall,
Hannah L. Turner,
Dennis R. Burton,
Andrew B. Ward
Posted 07 Nov 2019
bioRxiv DOI: 10.1101/833715 (published DOI: 10.1016/j.celrep.2020.02.061)
Posted 07 Nov 2019
Rational immunogen design aims to focus antibody responses to vulnerable sites on the primary antigens. Given the size of these antigens there is however potential for eliciting unwanted, off-target responses. Here, we used our electron microscopy polyclonal epitope mapping approach to describe the antibody specificities elicited by immunization of non-human primates with soluble HIV envelope trimers and subsequent repeated viral challenge. An increased diversity of epitopes recognized, and the approach angle by which these antibodies bound, constituted a hallmark of the humoral response in most protected animals. We also show that fusion peptide-specific antibodies are responsible for some neutralization breadth. Moreover, cryoEM analysis of a fully-protected animal revealed a high degree of clonality within a subset of putatively neutralizing antibodies, enabling a detailed molecular description of the antibody paratope. Our results provide important insights into the immune response against a vaccine candidate that entered into clinical trials earlier this year.
- Downloaded 522 times
- Download rankings, all-time:
- Site-wide: 41,539 out of 118,977
- In immunology: 1,050 out of 3,338
- Year to date:
- Site-wide: 37,328 out of 118,977
- Since beginning of last month:
- Site-wide: 90,436 out of 118,977
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!