Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,044 bioRxiv papers from 295,131 authors.

High throughput, error corrected Nanopore single cell transcriptome sequencing

By Kevin Lebrigand, Virginie Magnone, Pascal Barbry, Rainer Waldmann

Posted 05 Nov 2019
bioRxiv DOI: 10.1101/831495

Droplet-based high throughput single cell isolation techniques tremendously boosted the throughput of single cell transcriptome profiling experiments. However, those approaches only allow analysis of one extremity of the transcript after short read sequencing. We introduce an approach that combines Oxford Nanopore sequencing with unique molecular identifiers to obtain error corrected full length sequence information with the 10xGenomics single cell isolation system. This allows to examine differential RNA splicing and RNA editing at a single cell level.

Download data

  • Downloaded 2,128 times
  • Download rankings, all-time:
    • Site-wide: 2,177 out of 67,044
    • In genomics: 462 out of 4,558
  • Year to date:
    • Site-wide: 470 out of 67,044
  • Since beginning of last month:
    • Site-wide: 13 out of 67,044

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)