Northstar enables automatic classification of known and novel cell types from tumor samples
Bojk A. Berghuis,
Robert C. Jones,
Benedetta Nicolis di Robilant,
Rachel Yuan Nong Mikkelsen,
Michael F Clarke,
Stephen R. Quake
Posted 27 Oct 2019
bioRxiv DOI: 10.1101/820928 (published DOI: 10.1038/s41598-020-71805-1)
Posted 27 Oct 2019
Single cell transcriptomics is revolutionising our understanding of tissue and disease heterogeneity, yet cell type identificationl remains a partially manual task. Published algorithms for automatic cell annotation are limited to known cell types and fail to capture novel populations, especially cancer cells. We developed northstar, a computational approach to classify thousands of cells based on published data within seconds while simultaneously identifying and highlighting new cell states such as malignancies. We tested northstar on human glioblastoma and melanoma and obtained high accuracy and robustness. We collected eleven pancreatic tumors and identified three shared and five private neoplastic cell populations, offering insight into the origins of neuroendocrine and exocrine tumors. northstar is a useful tool to assign known and novel cell type and states in the age of cell atlases. ### Competing Interest Statement The authors have declared no competing interest.
- Downloaded 957 times
- Download rankings, all-time:
- Site-wide: 31,555
- In bioinformatics: 3,428
- Year to date:
- Site-wide: None
- Since beginning of last month:
- Site-wide: 112,425
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!