Rxivist logo

Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy

By Feng Wang, Zanlin Yu, Miguel Betegon, Melody Campbell, Tural Aksel, Jianhua Zhao, Sam Li, Shawn M. Douglas, Yifan Cheng, David Agard

Posted 22 Oct 2019
bioRxiv DOI: 10.1101/813972 (published DOI: 10.1016/j.jsb.2019.107437)

Cryo-EM samples prepared using the traditional methods often suffer from too few particles, poor particle distribution, or strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.

Download data

  • Downloaded 963 times
  • Download rankings, all-time:
    • Site-wide: 12,094 out of 88,877
    • In biophysics: 382 out of 3,870
  • Year to date:
    • Site-wide: 10,508 out of 88,877
  • Since beginning of last month:
    • Site-wide: 14,822 out of 88,877

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)