Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy
By
Feng Wang,
Zanlin Yu,
Miguel Betegon,
Melody Campbell,
Tural Aksel,
Jianhua Zhao,
Sam Li,
Shawn M. Douglas,
Yifan Cheng,
David A. Agard
Posted 22 Oct 2019
bioRxiv DOI: 10.1101/813972
(published DOI: 10.1016/j.jsb.2019.107437)
Cryo-EM samples prepared using the traditional methods often suffer from too few particles, poor particle distribution, or strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.
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