Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy
By
Feng Wang,
Zanlin Yu,
Miguel Betegon,
Melody Campbell,
Tural Aksel,
Jianhua Zhao,
Sam Li,
Shawn M. Douglas,
Yifan Cheng,
David Agard
Posted 22 Oct 2019
bioRxiv DOI: 10.1101/813972
(published DOI: 10.1016/j.jsb.2019.107437)
Cryo-EM samples prepared using the traditional methods often suffer from too few particles, poor particle distribution, or strongly biased orientation, or damage from the air-water interface. Here we report that functionalization of graphene oxide (GO) coated grids with amino groups concentrates samples on the grid with improved distribution and orientation. By introducing a PEG spacer, particles are kept away from both the GO surface and the air-water interface, protecting them from potential denaturation.
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