Rxivist logo

Prophase removal of chromosome-associated RNAs facilitates anaphase chromosome segregation

By Judith A. Sharp, Wei Wang, Michael D. Blower

Posted 21 Oct 2019
bioRxiv DOI: 10.1101/813527

During mitosis, the genome is transformed from a decondensed, transcriptionally active state to a highly condensed, transcriptionally inactive state. Mitotic chromosome reorganization is marked by the general attenuation of transcription on chromosome arms, yet how the cell regulates nuclear and chromatin-associated RNAs after chromosome condensation and nuclear envelope breakdown is unknown. SAF-A/hnRNPU is an abundant nuclear protein with RNA-to-DNA tethering activity, coordinated by two spatially distinct nucleic acid binding domains. Here we show that RNA is evicted from prophase chromosomes through Aurora-B-dependent phosphorylation of the SAF-A DNA-binding domain; failure to execute this pathway leads to accumulation of SAF-A:RNA complexes on mitotic chromosomes and elevated rates of anaphase segregation defects. This work reveals a role for Aurora-B in removing chromatin-associated RNAs during prophase, and demonstrates that Aurora-B dependent relocalization of SAF-A during cell division contributes to the fidelity of chromosome segregation.

Download data

  • Downloaded 431 times
  • Download rankings, all-time:
    • Site-wide: 37,121 out of 88,856
    • In cell biology: 1,791 out of 4,553
  • Year to date:
    • Site-wide: 23,584 out of 88,856
  • Since beginning of last month:
    • Site-wide: 12,796 out of 88,856

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News