Rxivist logo

Pinhole Optical Tweezers: Extending the Photobleaching Lifetime in the Presence of an Optical Trap by Wavefront Engineering

By Zheng Zhang, Joshua N. Milstein

Posted 17 Oct 2019
bioRxiv DOI: 10.1101/808352 (published DOI: 10.1016/j.bpj.2019.11.3327)

We present a new method for combining optical tweezers with single-molecule fluorescence in an engineered geometry we have coined a ‘pinhole’ optical trap. By utilizing an appropriately constructed Laguerre-Gaussian (LG) or ‘donut’ beam, and applying force along the axis of the trapping laser, one can maintain a low-intensity region of near-infrared (IR) light directly below the optical trap in which a biomolecule may be probed by both force spectroscopy and fluorescence. We show that within this region of low IR light intensity, the photobleaching lifetime of Alexa-647, an organic dye that is particularly sensitive to the high intensity trap light, can be significantly extended. This approach enables us to spatially separate the trap light from the fluorescence illumination without the need to physically separate, by many micrometers, the optical trap from the biological sample.

Download data

  • Downloaded 236 times
  • Download rankings, all-time:
    • Site-wide: 68,957 out of 100,488
    • In biophysics: 3,042 out of 4,425
  • Year to date:
    • Site-wide: 57,833 out of 100,488
  • Since beginning of last month:
    • Site-wide: None out of 100,488

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News

  • 20 Oct 2020: Support for sorting preprints using Twitter activity has been removed, at least temporarily, until a new source of social media activity data becomes available.
  • 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
  • 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
  • 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
  • 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
  • 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
  • 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
  • 22 Jan 2019: Nature just published an article about Rxivist and our data.
  • 13 Jan 2019: The Rxivist preprint is live!