Rxivist logo

Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement

By Avinash B Patel, Camille M Moore, Basil J. Greber, Jie Luo, Jeff Ranish, Eva Nogales

Posted 16 Oct 2019
bioRxiv DOI: 10.1101/804534 (published DOI: 10.7554/eLife.54449)

Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric Rsc8 dimer and built with the evolutionarily conserved subunits Sfh1, Rsc6, Rsc9 and Sth1. The flexible ATPase lobe, composed of helicase subunit Sth1, Arp7, Arp9 and Rtt102, is anchored through the interactions between the N-terminus of Sth1 and the core. Our cryo-EM analysis also shows that in addition to the expected nucleosome-Sth1 interactions, RSC engages histones and nucleosomal DNA through one arm of the core structure, composed of Rsc8 SWRIM domains, Sfh1 and Npl6. Our findings provide structural insights into the conserved assembly process for all members of the SWI/SNF family of remodelers, and illustrate how RSC selects, engages, and remodels nucleosomes.

Download data

  • Downloaded 875 times
  • Download rankings, all-time:
    • Site-wide: 14,149 out of 89,225
    • In molecular biology: 460 out of 3,022
  • Year to date:
    • Site-wide: 42,968 out of 89,225
  • Since beginning of last month:
    • Site-wide: 65,091 out of 89,225

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News