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An Improved Genome Assembly of the European Aspen Populus tremula

By Bastian Schiffthaler, Nicolas Delhomme, Carolina Bernhardsson, Jerry W Jenkins, Stefan Jansson, Par K Ingvarsson, Jeremy Schmutz, Nathaniel Robert Street

Posted 15 Oct 2019
bioRxiv DOI: 10.1101/805614

The genome assembly of the European aspen Populus tremula proved difficult for a short-read based strategy due to high genomic variation. As a consequence, the fragmented sequence is impeding studies that benefit from highly contiguous data, particularly genome-wide association studies (GWAS) and comparative genomics. Here we present an updated assembly based on long-read sequences, optical mapping and genetic mapping. This assembly - henceforth referred to as Potra V2 - is assembled into 19 contiguous chromosomes which provides a powerful tool for future association studies. The genome sequence and any feature files are available from the PopGenIE resource.

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