Rxivist logo

Revealing the molecular origins of fibrin’s elastomeric properties by in situ X-ray scattering

By Bart Eduard Vos, Cristina Martinez-Torres, Federica Burla, John W. Weisel, Gijsje H. Koenderink

Posted 08 Oct 2019
bioRxiv DOI: 10.1101/797464 (published DOI: 10.1016/j.actbio.2020.01.002)

Fibrin is an elastomeric protein forming highly extensible fiber networks that provide the scaffold of blood clots. Here we reveal the molecular mechanisms that explain the large extensibility of fibrin networks by performing in situ small angle X-ray scattering measurements while applying a shear deformation. We simultaneously measure shear-induced alignment of the fibers and changes in their axially ordered molecular packing structure. We show that fibrin networks exhibit distinct structural responses that set in consecutively as the shear strain is increased. They exhibit an entropic response at small strains (<5%), followed by progressive fiber alignment (>25% strain) and finally changes in the fiber packing structure at high strain (>100%). Stretching reduces the fiber packing order and slightly increases the axial periodicity, indicative of molecular unfolding. However, the axial periodicity changes only by 0.7%, much less than the 80% length increase of the fibers, indicating that fiber elongation mainly stems from uncoiling of the natively disordered αC-peptide linkers that laterally bond the molecules. Upon removal of the load, the network structure returns to the original isotropic state, but the fiber structure becomes more ordered and adopts a smaller packing periodicity compared to the original state. We conclude that the hierarchical packing structure of fibrin fibers, with built-in disorder, makes the fibers extensible and allows for mechanical annealing. Our results provide a basis for interpreting the molecular basis of haemostatic and thrombotic disorders associated with clotting and provide inspiration to design resilient bio-mimicking materials.

Download data

  • Downloaded 370 times
  • Download rankings, all-time:
    • Site-wide: 83,454
    • In biophysics: 3,099
  • Year to date:
    • Site-wide: 69,287
  • Since beginning of last month:
    • Site-wide: 125,872

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide