Rxivist logo

An inducible genome editing system for plants

By Xin Wang, Lingling Ye, Robertas Ursache, Ari Pekka Mähönen

Posted 23 Sep 2019
bioRxiv DOI: 10.1101/779140

Conditional manipulation of gene expression is a key approach to investigating the primary function of a gene in a biological process. While conditional and cell-type specific overexpression systems exist for plants, there are currently no systems available to disable a gene completely and conditionally. Here, we present a novel tool with which target genes can be efficiently conditionally knocked out at any developmental stage. The target gene is manipulated using the CRISPR-Cas9 genome editing technology, and conditionality is achieved with the well-established estrogen-inducible XVE system. Target genes can also be knocked-out in a cell-type specific manner. Our tool is easy to construct and will be particularly useful for studying genes which have null-alleles that are non-viable or show strong developmental defects.

Download data

  • Downloaded 1,806 times
  • Download rankings, all-time:
    • Site-wide: 4,291 out of 88,856
    • In plant biology: 63 out of 2,734
  • Year to date:
    • Site-wide: 4,288 out of 88,856
  • Since beginning of last month:
    • Site-wide: 5,868 out of 88,856

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News