Rxivist logo

The signal and the noise - characteristics of antisense RNA in complex microbial communities

By Thomas Yssing Michaelsen, Jakob Brandt, Caitlin Singleton, Rasmus H Kirkegaard, Nicola Segata, Mads Plesner Albertsen

Posted 20 Sep 2019
bioRxiv DOI: 10.1101/776971 (published DOI: 10.1128/mSystems.00587-19)

High-throughput sequencing has allowed unprecedented insight into the composition and function of complex microbial communities. With the onset of metatranscriptomics, it is now possible to interrogate the transcriptome of multiple organisms simultaneously to get an overview of the gene expression of the entire community. Studies have successfully used metatranscriptomics to identify and describe relationships between gene expression levels and community characteristics. However, metatranscriptomic datasets contain a rich suite of additional information which is just beginning to be explored. In this minireview we discuss the different computational strategies for handling antisense expression in metatranscriptomic samples and highlight their potentially detrimental effects on downstream analysis and interpretation. We also surveyed the antisense transcriptome of multiple genomes and metagenome-assembled genomes (MAGs) from five different datasets and found high variability in the level of antisense transcription for individual species which were consistent across samples. Importantly, we tested the hypothesis that antisense transcription is primarily the product of transcriptional noise and found mixed support, suggesting that the total observed antisense RNA in complex communities arises from a compounded effect of both random, biological and technical factors. Antisense transcription can provide a rich set of information, from technical details about data quality to novel insight into the biology of complex microbial communities.

Download data

  • Downloaded 239 times
  • Download rankings, all-time:
    • Site-wide: 98,919
    • In microbiology: 7,197
  • Year to date:
    • Site-wide: 134,195
  • Since beginning of last month:
    • Site-wide: 133,830

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)